uterine smooth muscle utsmc cell line  (PromoCell)

Journal: Molecular Human Reproduction

Article Title: Crosstalk between monocytes and myometrial smooth muscle in culture generates synergistic pro-inflammatory cytokine production and enhances myocyte contraction, with effects opposed by progesterone

doi: 10.1093/molehr/gav027

Figure Lengend Snippet: Secretions of IL-6 and IL-8 were enhanced from UtSMCs, in the presence of primary monocytes, compared to either cell type alone, in the presence or absence of LPS. Uterine smooth muscle cells (UtSMCs) were cultured alone (1 × 10 5 cells), in the presence of primary monocytes from term pregnant women, in a 10:1 ratio, or primary monocytes were cultured in isolation (1 × 10 4 cells). Cells were treated with 100 ng/ml LPS and supernatants were harvested after 24 h and assayed by ELISA for ( A ) IL-6 and ( B ) IL-8. LPS up-regulated secretion from UtSMCs and UtSMC/monocyte co-culture but not monocytes cultured alone, for both IL-6 and IL-8. Comparison of the additive secretion from UtSMCs and primary monocytes independently cultured (spotted bars), with UtSMC/monocyte co-culture (solid bars) for ( C ) IL-6, found the co-culture had significantly higher secretion of IL-6 both alone and after LPS treatment, whereas for ( D ) IL-8, only after LPS treatment, was the co-culture secretion greater than additive secretion. Data shown are n = 5 experiments, and are shown as mean ± SEM. Data were analysed by two-way ANOVA and post hoc Tukey or Bonferroni multiple comparisons, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns, non-significant.

Article Snippet: Primary uterine smooth muscle cells (UtSMCs) were obtained from Lonza (Slough, UK), and were derived from a non-pregnant donor by enzymatic dispersion.

Techniques: Cell Culture, Isolation, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Comparison